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KMID : 0380020190340040346
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 4 p.346 ~ p.351
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Optimization of Extraction Conditions for Astaxanthin from Haematococcus pluvialis
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Kim Song-Yi
Jung Sung-Jin
Kim Ho-Seob
Gam Da-Hye
Kim Jin-Woo
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Abstract
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In this study, the selection of extraction solvent and optimization of extraction condition were carried out to increase the extraction of astaxanthin, an antioxidant material produced from Haematococcus pluvialis. For increasing the extraction of astaxanthin through more effective cell disruption, the concentration of acetone, rotation speed of homogenizer, extraction temperature, and extraction time were sequentially optimized. When acetone (50% v/v) was used as an extract solvent, the extraction concentration of astaxanthin was found to be the highest (1.41 μg/mL), and the extraction concentration increased 3.3 times compared to ethanol extraction. As the concentration of acetone increased to 99.5% (v/v), it was found that the concentration of astaxanthin increased proportionally to 2.47 μg/mL. When evaluating the effect of rotation speed and extraction temperature, astaxanthin concentration was maximized to 3.93 g/mL at 15,318 rpm (impeller tip speed = 5.67 m/s) and 52.5oC. In addition, as extraction time increased, the concentration of extract was increased and resulted in a maximum of 4.76 μg/mL at 16.6 min which was a 10.9-fold increase compared to ethanol extraction indicating that acetone is more effective than conventional solvents in the extraction of astaxanthin.
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KEYWORD
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Astaxanthin, Haematococcus pluvialis, extraction, optimization
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